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KLHL13 monoclonal antibody

货号: MB21342

价格：￥ 1200

货期：现货（1-2个工作日）

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No. :MB21342

Product Name :KLHL13 monoclonal antibody

Swiss-Prot :Q9P2N7

Host :Mouse

Reactivity :Human

Applications :WB,IHC,IF,FCM

Application_all :WB:1/500 - 1/2000 IHC:1/200 - 1/1000 IF:1/200 - 1/1000 FC:1/200 - 1/400

Background :KLHL13 (kelch-like 13), also known as BKLHD2 (BTB and kelch domain-containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate- specific adapter of an E3 ubiquitin-protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL-3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin-mediated proteolysis. Via its BTB and C-terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL-3 ligase.

Product :Ascitic fluid containing 0.03% sodium azide.

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Mouse IgG1

BiowMW :74kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BKLHD2; FLJ10262; MGC74791; KLHL13

Immunogen :Purified recombinant fragment of human KLHL13 expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis using KLHL13 mouse mAb against Hela (1) and MCF-7 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded human brain tissues (left) and pancreas tissues (right) using KLHL13 mouse mAb with DAB staining.

Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of 3T3/L1 cells using KLHL13 mouse mAb (green) and negative control (purple).