产品类别

联系我们

电话:18013895086

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PIF1 polyclonal antibody

货号: BS60114

价格：￥

货期：现货（1-2个工作日）

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS60114

Product Name :PIF1 polyclonal antibody

Swiss-Prot :Q9H611

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first. This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae. The PIF1 gene is highly conserved from yeast to humans. Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation. Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity. PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PIF1 polyclonal antibody detects endogenous levels of PIF1 protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ATP-dependent DNA helicase PIF1; DNA repair and recombination helicase PIF-1; PIF1/RRM3 DNA helicase-like protein; C15orf20

Immunogen :A synthetic peptide corresponding to residues in Human PIF1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PIF1 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate