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VE-Cadherin (phospho-Y731) polyclonal antibody

货号: BS4242

价格：￥

货期：现货（1-2个工作日）

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No. :BS4242

Product Name :VE-Cadherin (phospho-Y731) polyclonal antibody

Swiss-Prot :P33151

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The cadherins are a family of Ca 2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including β-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-VE-Cadherin (Y731) polyclonal antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Tyr731

BiowMW :~ 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; VE-cadherin; CD144; CDH5; Cadherin 5

Immunogen :Synthetic phosphopeptide derived from human VE-Cadherin around the phosphorylation site of Tyrosine 731.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-VE-Cadherin (Y731) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:H9C2 cell lysate treated with EGF(0.1ng/ml,30mins)

Immunohistochemistry (IHC) analyzes of p-VE-Cadherin (Y731) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

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