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RBFOX1 polyclonal antibody

货号: BS78926

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS78926

Product Name :RBFOX1 polyclonal antibody

Swiss-Prot :Q9NWB1

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:100 - 1:200

Background :The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :45-50KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RBFOX1;2BP1;A2BP1;FOX-1;FOX1;HRNBP1

Immunogen :Recombinant fusion protein of human RBFOX1(NP_665900.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using RBFOX1 antibody at 1:849 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of mouse brain cells using RBFOX1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat brain cells using RBFOX1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.