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POLR2J polyclonal antibody

货号: BS7200

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS7200

Product Name :POLR2J polyclonal antibody

Swiss-Prot :P52435

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :13kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :POLR2J;POLR2J1;RPB11;RPB11A;RPB11m;hRPB14

Immunogen :Recombinant fusion protein of human POLR2J(NP_006225.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of mouse skeletal muscle, using POLR2J antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of A549 cells using POLR2J antibody .