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PCK1 polyclonal antibody

货号: BS6870

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6870

Product Name :PCK1 polyclonal antibody

Swiss-Prot :P35558

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :69KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PCK1;PEPCK-C;PEPCK1;PEPCKC

Immunogen :Recombinant fusion protein of human PCK1(NP_002582.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PCK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of HepG2 cells, using PCK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunofluorescence analysis of rat kidney using PCK1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human kidney cancer using PCK1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Repurposing Doxepin to Ameliorate Steatosis and Hyperglycemia by Activating FAM3A Signaling Pathway

JournalDiabetes

ApplicationWB

IF7.72

SpeciesMouse

PMID32312868
PCK1 negatively regulates cell cycle progression and hepatoma cell proliferation via the AMPK/p27 Kip1 axis

JournalJournal Of Experimental & Clinical Cancer Research

ApplicationWB

IF7.068

SpeciesHuman

PMID30717766
PCK1 Downregulation Promotes TXNRD1 Expression and Hepatoma Cell Growth via the Nrf2/Keap1 Pathway

JournalFrontiers in Oncology

ApplicationWB

IF4.848

SpeciesHuman

PMID30619751
Transcriptomic changes associated with PCK1 overexpression in hepatocellular carcinoma cells detected by RNA-seq

JournalGenes & Diseases

ApplicationWB

IF4.803

SpeciesMouse,Human

PMID32181286
FAM3B (PANDER) functions as a co‐activator of FOXO1 to promote gluconeogenesis in hepatocytes

JournalJournal Of Cellular And Molecular Medicine

ApplicationWB

IF4.658

SpeciesMouse,Human

PMID30488666