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PCK1 polyclonal antibody

货号: BS6870

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6870

Product Name :PCK1 polyclonal antibody

Swiss-Prot :P35558

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:1000 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :69KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PCK1;PEPCK-C;PEPCK1;PEPCKC

Immunogen :Recombinant fusion protein of human PCK1(NP_002582.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PCK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of HepG2 cells, using PCK1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Immunofluorescence analysis of rat kidney using PCK1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human kidney cancer using PCK1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Dapagliflozin Aggravates Renal Injury via Promoting Gluconeogenesis in db/db Mice

JournalCellular Physiology And Biochemistry

ApplicationWB

IF0

SpeciesMouse

PMID29495021
Genistein accelerates glucose catabolism via activation the GPER-mediated cAMP/PKA-AMPK signaling pathway in broiler chickens

JournalLIFE SCIENCES

ApplicationWB

IF5.037

SpeciesChicken

PMID35640778
Imipramine activates FAM3A-FOXA2-CPT2 pathway to ameliorate hepatic steatosis

JournalMETABOLISM-CLINICAL AND EXPERIMENTAL

ApplicationWB

IF13.934

SpeciesMouse

PMID35995281
FAM3A maintains metabolic homeostasis by interacting with F1-ATP synthase to regulate the activity and assembly of ATP synthase

JournalMETABOLISM-CLINICAL AND EXPERIMENTAL

ApplicationWB

IF13.934

SpeciesMouse

PMID36470472
PCK1 attenuates tumor stemness via activating the Hippo signaling pathway in hepatocellular carcinoma

JournalGenes & Diseases

ApplicationWB

IF6.8

SpeciesHuman

PMID