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PCNA polyclonal antibody

货号: BS6438

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6438

Product Name :PCNA polyclonal antibody

Swiss-Prot :P12004

Host :Rabbit

Reactivity :Human, Mouse, Rat,African green monkey

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :34KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PCNA;ATLD2

Immunogen :A synthetic peptide of human PCNA(NP_002583.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PCNA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

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ApplicationIF

IF3.323

SpeciesMouse

PMID23884141
Dietary cholesterol promotes AOM-induced colorectal cancer through activating the NLRP3 inflammasome

JournalBiochemical Pharmacology

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IF4.96

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Pre-treatment of human umbilical cord-derived mesenchymal stem cells with interleukin-6 abolishes their growth-promoting effect on gastric cancer cells

JournalInternational Journal Of Molecular Medicine

ApplicationIF,IHC

IF3.098

SpeciesHuman,Mouse

PMID25483835
Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

JournalOncology Reports

ApplicationWB

IF3.041

SpeciesRat

PMID27277008
Glycolysis inhibitor 2-deoxy-D-glucose suppresses carcinogen-induced rat hepatocarcinogenesis by restricting cancer cell metabolism

JournalMolecular Medicine Reports

ApplicationIHC,WB

IF2.1

SpeciesRat

PMID25394852