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HIF-1α (K377) polyclonal antibody（KU）

货号: BS3514

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS3514

Product Name :HIF-1α (K377) polyclonal antibody（KU）

Swiss-Prot :Q16665

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP

Application_all :WB: 1:1000~1:2000 IP: 1:50~1:200

Background :Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, Arnt 1 (also designated HIF-1β), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HIF-1α (K377) polyclonal antibody detects endogenous levels of HIF-1α protein.

BiowMW :~ 120 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hypoxia Signaling,Warburg Effect,Glutamine Metabolism,MTOR signaling,

Alternative Name :Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A; BHLHE78, MOP1

Immunogen :Synthetic peptide, corresponding to Human HIF-1α.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of HIF-1α (K377) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)

Western blot (WB) analysis of HIF-1α (K377) polyclonal antibody at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug)

Immunoprecipitation of A549 cell lysates using HIF-1α (K377) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HIF-1α (K377) pAb #BS3514.

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