产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

HIF-1α (K377) polyclonal antibody（KU）

货号: BS3514

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS3514

Product Name :HIF-1α (K377) polyclonal antibody（KU）

Swiss-Prot :Q16665

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP

Application_all :WB: 1:1000~1:2000 IP: 1:50~1:200

Background :Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, Arnt 1 (also designated HIF-1β), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :HIF-1α (K377) polyclonal antibody detects endogenous levels of HIF-1α protein.

BiowMW :~ 120 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hypoxia Signaling,Warburg Effect,Glutamine Metabolism,MTOR signaling,

Alternative Name :Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A; BHLHE78, MOP1

Immunogen :Synthetic peptide, corresponding to Human HIF-1α.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of HIF-1α (K377) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:EC9706 whole cell lysate(40ug)

Western blot (WB) analysis of HIF-1α (K377) polyclonal antibody at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug)

Immunoprecipitation of A549 cell lysates using HIF-1α (K377) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using HIF-1α (K377) pAb #BS3514.

Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner

JournalMolecular Cancer

ApplicationWB

IF27.401

SpeciesHuamn

PMID
α-Mangostin inhibits hypoxia-driven ROS-induced PSC activation and pancreatic cancer cell invasion

JournalCancer Letters

ApplicationWB

IF8.679

SpeciesHuman

PMID
Curcumin attenuates angiogenesis in liver fibrosis and inhibits angiogenic properties of hepatic stellate cells

JournalJournal Of Cellular And Molecular Medicine

ApplicationWB

IF4.486

SpeciesRat

PMID
Steroid Receptor Coactivator-3 Regulates Glucose Metabolism in Bladder Cancer Cells through Coactivation of Hypoxia Inducible Factor 1α

JournalJournal Of Biological Chemistry

ApplicationWB

IF4.106

SpeciesHuman

PMID24584933
TJ0711, a novel vasodilatory β-blocker, protects SHR rats against hypertension induced renal injury

JournalAmerican Journal of Translational Research

ApplicationIMMUNOSTAINING AND HISTOLOGICAL,WB

IF4.06

SpeciesRat

PMID23634239