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Bax (S4) polyclonal antibody（KU）

货号: BS1030

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1030

Product Name :Bax (S4) polyclonal antibody（KU）

Swiss-Prot :Q07812

Host :Rabbit

Reactivity :Human

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Bax (S4) polyclonal antibody detects endogenous levels of Bax protein.

BiowMW :~ 21 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,SAPK JNK Signaling Cascades,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4

Immunogen :Synthetic peptide, corresponding to the N-terminus of Human Bax.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Bax (S4) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Bax (S4) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Immunoprecipitation of A549 cell lysates using Bax (S4) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using Bax (S4) pAb #BS1030.

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