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Smad5 (C-terminus) monoclonal antibody

货号: MB0069

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0069

Product Name :Smad5 (C-terminus) monoclonal antibody

Swiss-Prot :Q99717

Host :Mouse

Reactivity :Human,Mouse

Applications :WB ICC FC

Application_all :WB: 1:1000 ICC: 1:50~100 FC: 1:50~100

Background :Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of SMAD5 (C-terminus) and does not cross-react with related proteins

BiowMW :Predicted band size:55KDa Observed band size:55KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5

Immunogen :Synthetic peptide, corresponding to the C-terminus of Human Smad5.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of SMAD5 antibody in Mouse testis cell lysates using SMAD5 antibody (1:1000 diluted) .

Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.

Immunocytochemistry of HeLa cells using anti- SMAD5 (C-terminus) antibody diluted 1:75.