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RANKL polyclonal antibody

货号: BS72037

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS72037

Product Name :RANKL polyclonal antibody

Swiss-Prot :O14788

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB,IF/ICC,ELISA

Application_all :WB 1:500 - 1:1000; IF 1:50 - 1:200

Background :This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human RANKL

BiowMW :~ 32 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD254; ODF; OPGL; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2; sOdf

Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 78-317 of human RANKL

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RANKL antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human colon using RANKL Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of Jurkat cells using RANKL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

The Antioxidant Property of Pachymic Acid Improves Bone Disturbance against AH Plus–induced Inflammation in MC-3T3 E1 Cells

JournalJournal Of Endodontics

ApplicationWB

IF4.171

SpeciesMouse

PMID
Metformin stimulates osteoprotegerin and reduces RANKL expression in osteoblasts and ovariectomized rats

JournalJournal Of Cellular Biochemistry

ApplicationWB

IF3.448

SpeciesRat

PMID21618594
Metformin Rescues the MG63 Osteoblasts against the Effect of High Glucose on Proliferation

JournalJournal of Diabetes Research

ApplicationWB

IF2.965

SpeciesHuman

PMID24812633
Risedronate inhibits bone marrow mesenchymal stem cell adipogenesis and switches RANKL/OPG ratio to impair osteoclast differentiation

JournalJournal Of Surgical Research

ApplicationWB

IF1.841

SpeciesHuman

PMID22487394
Enhancement of osteogenesis post-splenectomy does not attenuate bone loss in ovariectomized rats

JournalJournal Of Orthopaedic Research

ApplicationIHC

IF3.494

SpeciesRat

PMID25640957