产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PARP-1 (D214) polyclonal antibody

货号: BS7047

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS7047

Product Name :PARP-1 (D214) polyclonal antibody

Swiss-Prot :P09874

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PARP-1 (D214) polyclonal antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage.

BiowMW :~ 89, 116 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,

Alternative Name :Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT; PPOL

Immunogen :Synthetic peptide, corresponding to amino acids 180-214 of Human PARP-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PARP-1 (D214) polyclonal antibody at 1:1000 dilution Lane1:NIH-3T3 whole cell lysate(20ug) Lane2:NIH-3T3 treated with PMA (60ng/ml, 5min) whole cell lysate (20ug) Lane3:NIH-3T3 treated with PMA (60ng/ml, 15min) whole cell lysate (20ug) Lane4:NIH-3T3 treated with PMA (60ng/ml, 30min) whole cell lysate (20ug)

Synergistic effect of arginine-specific ADP-ribosyltransferase 1 and poly(ADP-ribose) polymerase-1 on apoptosis induced by cisplatin in CT26 cells

JournalOncology Reports

ApplicationWB

IF3.041

SpeciesMouse

PMID24676941
Inhibition of arginine ADP-ribosyltransferase 1 reduces the expression of poly(ADP-ribose) polymerase-1 in colon carcinoma

JournalInternational Journal Of Molecular Medicine

ApplicationWB

IF2.928

SpeciesMouse

PMID23652727
IGF-1 gene-modified muscle-derived stem cells are resistant to oxidative stress via enhanced activation of IGF-1R/PI3K/AKT signaling and secretion of VEGF

JournalMolecular And Cellular Biochemistry

ApplicationWB

IF2.795

SpeciesRat

PMID
FoxM1 Directs STAT3 Expression Essential for Human Endometrial Stromal Decidualization

JournalScientific Reports

ApplicationWB

IF3.998

SpeciesHuman

PMID26334131
M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus

JournalPLoS One

ApplicationWB

IF2.776

SpeciesRat

PMID27870896