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PARP-1 (D214) polyclonal antibody

货号: BS7047

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS7047

Product Name :PARP-1 (D214) polyclonal antibody

Swiss-Prot :P09874

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PARP-1 (D214) polyclonal antibody detects endogenous levels of full length PARP1 (116 kDa), as well as the large fragment (89 kDa) of PARP1 resulting from caspase cleavage.

BiowMW :~ 89, 116 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,

Alternative Name :Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1; PARP1; ADPRT; PPOL

Immunogen :Synthetic peptide, corresponding to amino acids 180-214 of Human PARP-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PARP-1 (D214) polyclonal antibody at 1:1000 dilution Lane1:NIH-3T3 whole cell lysate(20ug) Lane2:NIH-3T3 treated with PMA (60ng/ml, 5min) whole cell lysate (20ug) Lane3:NIH-3T3 treated with PMA (60ng/ml, 15min) whole cell lysate (20ug) Lane4:NIH-3T3 treated with PMA (60ng/ml, 30min) whole cell lysate (20ug)

Matrine‑induced apoptosis in Hep3B cells via the inhibition of MDM2

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesHuman

PMID27959389
Tubb3 regulation by the Erk and Akt signaling pathways: a mechanism involved in the effect of arginine ADP-ribosyltransferase 1 (Art1) on apoptosis of colon carcinoma CT26 cells

JournalTumor Biology

ApplicationWB

IF0

SpeciesMouse

PMID26373733
-Hederin Arrests Cell Cycle at G2/M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-B Signaling in Colon Cancer Cells

JournalBiomed Research International

ApplicationWB

IF2.197

SpeciesHuman

PMID30363706