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MBTPS2 polyclonal antibody

货号: BS61210

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS61210

Product Name :MBTPS2 polyclonal antibody

Swiss-Prot :O43462

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The transcription factors SREBPs (sterol regulatory element binding proteins) span the ER membrane and, in response to sterol depletion, the N-terminal domain of SREBPs are proteolytically activated, released from the membrane and then translocate to the nucleus where they induce the expression of genes regulating cholesterol metabolism. This proteolytic activation requires the sequential cleavage of SREBPs at Site-1, within the lumen of the ER, followed by cleavage at Site-2, within the first transmembrane domain. The cleavage at Site-1 separates the N-terminal and C-terminal domains of the protein and it requires the serine protease, S1P (Site-1 protease). Site-2 is subsequently processed by a putative zinc metalloprotease S2P, which releases the activated N-terminal domain for nuclear translocation. This proteolytic pathway is tightly regulated by sterol levels and is under the control of SCAP (SREBP cleavage-activating protein). SCAP, a sterol sensor, is latently bound to the C-terminal regulatory domains of the SREBPs, and it regulates cleavage of SREBPs at Site-1. Sterol levels influence the activity of SCAP, as SCAP is activated only in sterol-depleted cells and it inhibited by sterol accumulation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MBTPS2 polyclonal antibody detects endogenous levels of MBTPS2 protein.

BiowMW :~ 57 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Membrane-bound transcription factor site-2 protease (EC:3.4.24.85); Endopeptidase S2P; Sterol regulatory element-binding proteins intramembrane protease; SREBPs intramembrane protease; MBTPS2; S2P

Immunogen :A synthetic peptide corresponding to residues in Human MBTPS2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MBTPS2 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264.7 whole cell lysate

Site-1 protease controls osteoclastogenesis by mediating LC3 transcription

JournalCell Death And Differentiation

ApplicationWB

IF15.828

SpeciesMouse

PMID33469231