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PKLR polyclonal antibody

货号: BS60443

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS60443

Product Name :PKLR polyclonal antibody

Swiss-Prot :P30613

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :In mammals, four different isoenzymes exist for pyruvate kinase. Based on their tissue distribution, the isoenzymes are designated L-type (for predominant expression in the liver), R-type (for predominant expression in red blood cells), M1-type (for predominant expression in muscle, brain and heart) and M2-type (for predominant expression in fetal tissues). Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKLR (pyruvate kinase, liver and RBC) gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters. The R-type isoform, also known as RPK (R-type pyruvate kinase), exists as a tetramer and, when functioning improperly, can result in chronic/hereditary nonspherocytic hemolytic anemia (CNSHA/HNSHA) or pyruvate kinase hyperactivity (also called high red cell ATP syndrome). The L-type isoform, alternatively known as PKL (pyruvate kinase L-type), also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young (MODY).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKLR polyclonal antibody detects endogenous levels of PKLR protein.

BiowMW :~ 62 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Pyruvate kinase PKLR; Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase; PKLR; PK1, PKL

Immunogen :A synthetic peptide corresponding to residues in Human PKLR.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PKLR polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate