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TNF-α polyclonal antibody

货号: BS6000

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6000

Product Name :TNF-α polyclonal antibody

Swiss-Prot :P01375

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :IHC IF

Application_all :IHC 1:50 - 1:200 IF 1:50 - 1:100

Background :Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 a band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 a protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TNF-α polyclonal antibody detects endogenous levels of TNF-α protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Necrotic Cell Death,

Alternative Name :Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, soluble form; TNF; TNFA; TNFSF2; TNFα; TNF α

Immunogen :A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TNF (NP_000585.2).

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry of paraffin-embedded human thyroid cancer using TNF-α pAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L929 cells using TNF alpha antibody at dilution of 1:100.

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