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TNF-α polyclonal antibody

货号: BS6000

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6000

Product Name :TNF-α polyclonal antibody

Swiss-Prot :P01375

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :IHC IF

Application_all :IHC 1:50 - 1:200 IF 1:50 - 1:100

Background :Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 a band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 a protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TNF-α polyclonal antibody detects endogenous levels of TNF-α protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Necrotic Cell Death,

Alternative Name :Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, soluble form; TNF; TNFA; TNFSF2; TNFα; TNF α

Immunogen :A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TNF (NP_000585.2).

Conjugate :Unconjugated

Modification :Unmodification

Immunohistochemistry of paraffin-embedded human thyroid cancer using TNF-α pAb at dilution of 1:100 (40x lens).

Immunofluorescence analysis of L929 cells using TNF alpha antibody at dilution of 1:100.

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IF1.813

SpeciesMouse

PMID26101535
Nanoparticle-mediated intravesical delivery of conditioned medium derived from mesenchymal stem cells for interstitial cystitis/bladder pain syndrome treatment

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SpeciesRat

PMID
AICAR, an AMP-Activated Protein Kinase Activator, Ameliorates Acute Pancreatitis-Associated Liver Injury Partially Through Nrf2-Mediated Antioxidant Effects and Inhibition of NLRP3 Inflammasome Activation

JournalFrontiers in Pharmacology

ApplicationIHC

IF5.81

SpeciesRat

PMID34531748
7-Hydroxycoumarin mitigates the severity of collagen-induced arthritis in rats by inhibiting proliferation and inducing apoptosis of fibroblast-like synoviocytes via suppression of Wnt/β-catenin signaling pathway

JournalPhytomedicine

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IF5.34

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PMID34752968
Lung adenocarcinoma‐related TNF‐α‐dependent inflammation upregulates MHC‐II on alveolar type II cells through CXCR‐2 to contribute to Treg expansion

JournalFaseb Journal

ApplicationIHC,IF

IF4.966

SpeciesHuman,Mouse

PMID33000506