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SLU7 polyclonal antibody（KU）

货号: BS5936

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS5936

Product Name :SLU7 polyclonal antibody（KU）

Swiss-Prot :O95391

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :In order to produce correctly spliced messenger RNA, two catalytic splicing steps are required. After catalytic step I, a major remodeling of the spliceosome occurs to establish the active site for step II. During the second step of mRNA splicing, exon 1 attacks an adenine-guanine (AG) dinucleotide at the 3' splice site. SLU7, the human homolog of the yeast step II splice factor Slu7, is required for selection of the correct AG. Human SLU7 associates with the spliceosome late in the splicing pathway prior to recognition of the 3' splice site for step II. SLU7 depletion in HeLa nuclear extract reveals that SLU7 is required to hold exon 1 tightly within the spliceosome for attack on a prespecified AG.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SLU7 polyclonal antibody detects endogenous levels of SLU7 protein.

BiowMW :~ 68 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Pre-mRNA-splicing factor SLU7; hSlu7

Immunogen :Synthetic peptide, corresponding to amino acids 154-199 of Human SLU7.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SLU7 (F144) pAb at 1:1000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:L02 whole cell lysate(40ug)