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PCB polyclonal antibody

货号: BS5841

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS5841

Product Name :PCB polyclonal antibody

Swiss-Prot :P11498

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Pyruvate carboxylase (PCB) is a biotinylated mitchondrial enzyme that catalyzes the synthesis of oxaloacetate from pyruvate in a tissue specific manner. In addition to covalently binding the biotin cofactor, PCB contains consensus sequences for the attachment of ATP and the substrate pyruvate. The PCB gene is located on the long arm of chromosome 11. Mutations in PCB metabolism (pyruvate carboxylase deficiency) are known to cause lactic acidosis, hypoglycemia and mental retardation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PCB polyclonal antibody detects endogenous levels of PCB protein.

BiowMW :~ 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase; PCB; PC

Immunogen :Synthetic peptide, corresponding to amino acids 400-445 of Human PCB.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PCB polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate Lane2:NIH-T3 cell lysate Lane3:H9C2 cell lyate

Immunohistochemistry (IHC) analyzes of PCB pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.