产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Casein Kinase Iα (phospho-Y321) polyclonal antibody （KU）

货号: BS4602

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4602

Product Name :Casein Kinase Iα (phospho-Y321) polyclonal antibody （KU）

Swiss-Prot :P48729

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIα, β, γ, e and δ, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The a catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Casein Kinase Iα (Y321) pAb detects endogenous levels of Casein Kinase Iα protein only when phosphorylated at Tyr321.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,

Alternative Name :CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1; CKIα; CK-Iα; CKI-α; CK Iα

Immunogen :Synthetic phosphopeptide derived from human Casein Kinase Iα around the phosphorylation site of Tyrosine 321

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Casein Kinase Iα (Y321) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40µg) Lane2:L02 whole cell lysate(40µg) Lane3:The Brain tissue lysate of Mouse(40µg) Lane4:The Brain tissue lysate of Rat(40µg)