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Rsk-1 (phospho-T359/S363) polyclonal antibody（KU）

货号: BS4276

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4276

Product Name :Rsk-1 (phospho-T359/S363) polyclonal antibody（KU）

Swiss-Prot :Q15418

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IF IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200

Background :The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rsk-1 (phospho-T359/S363) polyclonal antibody detects endogenous levels of Rsk-1 protein only when phosphorylated at Thr359/Ser363.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; RPS6KA1; MAPKAPK1A; RSK1; S6K-α1; S6K α1; S6Kα1;

Immunogen :Synthetic phosphopeptide derived from human Rsk-1 around the phosphorylation site of Thr359/Ser363.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Rsk-1 (T359/S363) pAb at 1:2000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug)

Immunofluorescence analysis of CT26 cells using Rsk-1 (phospho-T359/S363) antibody at dilution of 1:50.

Immunoprecipitation of HepG2 cell lysate using p-Rsk-1 (T359/S363) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using p-Rsk-1 (T359/S363) #BS4276. “↑”（supernatant）; “↓(deposition）