Product Name :NOS1 (phospho-S852) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC: 1:50~1:200
Background :NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-NOS1 (S852) polyclonal antibody detects endogenous levels of human NOS1 protein only when phosphorylated at Ser852. This antibody also detects mouse and rat NOS1 protein when phosphorylated at the corresponding sites (Ser847).
BiowMW :~ 43,125,149,160 kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Nitric oxide synthase, brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS; NOS1
Immunogen :Synthetic phosphopeptide derived from human NOS1 around the phosphorylation site of Serine 852.
Modification :Phosphorylation
