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AKT (phospho-T450) polyclonal antibody（KU）

货号: BS4255

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4255

Product Name :AKT (phospho-T450) polyclonal antibody（KU）

Swiss-Prot :P31749/P31751/Q9Y243

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200 IF: 1:100~1:500

Background :AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-AKT (T450) polyclonal antibody detects endogenous levels of AKT1 only when phosphorylated at Thr450. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ

Immunogen :Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 450.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of AKT (phospho-T450) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF(0.1ng/ml,5min) whole cell lysate Lane3:L02 treated with EGF(0.1ng/ml,10min) whole cell lysate Lane4:L02 treated with EGF(0.1ng/ml,15min) whole cell lysate

Immunohistochemistry (IHC) analyzes of p-AKT (T450) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunofluorescence analysis of NIH-3T3 cells using AKT (phospho-T450) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of 3T3-L1 cell lysates using AKT (phospho-T450) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using AKT (phospho-T450) pAb.