产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Smad2 (phospho-S467) polyclonal antibody（KU）

货号: BS4172

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4172

Product Name :Smad2 (phospho-S467) polyclonal antibody（KU）

Swiss-Prot :Q15796

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:100~1:500

Background :Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Smad2 (S467) polyclonal antibody detects endogenous levels of Smad2 protein only when phosphorylated at Ser467.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Immunogen :Synthetic phosphopeptide derived from human Smad2 around the phosphorylation site of Serine 467.

Conjugate :Unconjugated

Modification :Phosphorylated

Immunohistochemistry (IHC) analyzes of p-Smad2 (S467) pAb in paraffin-embedded human breast cancer tissue.

Immunofluorescence analysis of HepG2 cells using Smad2 (phospho-S467) pAb at dilution of 1:200 (40x lens).

Anti‑fibrotic effects of Acremoniumterricola milleretal mycelium on immunological hepatic fibrosis in rats

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesRat

PMID25270983
Astragalus and Paeoniae radix rubra extract inhibits liver fibrosis by modulating the transforming growth factor‑β/Smad pathway in rats

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesRat

PMID25373883
TGF-β induces M2-like macrophage polarization via SNAIL-mediated suppression of a pro-inflammatory phenotype

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID27418133
FHL3 links cell growth and self-renewal by modulating SOX4 in glioma

JournalCell Death And Differentiation

ApplicationWB

IF8.086

SpeciesHuman

PMID29955125
Epithelial and interstitial Notch1 activity contributes to the myofibroblastic phenotype and fibrosis

JournalCell Communication and Signaling

ApplicationWB

IF5.111

SpeciesRat

PMID31718671