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Smad2 (phospho-S467) polyclonal antibody（KU）

货号: BS4172

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS4172

Product Name :Smad2 (phospho-S467) polyclonal antibody（KU）

Swiss-Prot :Q15796

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:100~1:500

Background :Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Smad2 (S467) polyclonal antibody detects endogenous levels of Smad2 protein only when phosphorylated at Ser467.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Immunogen :Synthetic phosphopeptide derived from human Smad2 around the phosphorylation site of Serine 467.

Conjugate :Unconjugated

Modification :Phosphorylated

Immunohistochemistry (IHC) analyzes of p-Smad2 (S467) pAb in paraffin-embedded human breast cancer tissue.

Immunofluorescence analysis of HepG2 cells using Smad2 (phospho-S467) pAb at dilution of 1:200 (40x lens).

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PMID23338277
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Hydrogen Sulfide Donor GYY4137 Protects against Myocardial Fibrosis

JournalOxidative Medicine and Cellular Longevity

ApplicationWB

IF6.543

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Curcumin inhibits cobalt chloride-induced epithelial-to-mesenchymal transition associated with interference with TGF- β /Smad signaling in hepatocytes

JournalLaboratory Investigation

ApplicationWB

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SpeciesRat,Human

PMID26302188
Sodium Tanshinone IIA Sulfonate Ameliorates Bladder Fibrosis in a Rat Model of Partial Bladder Outlet Obstruction by Inhibiting the TGF-β/Smad Pathway Activation

JournalPLoS One

ApplicationIHC

IF2.776

SpeciesRat

PMID26061047