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p53 (phospho-S15) polyclonal antibody（KU）

货号: BS4144

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4144

Product Name :p53 (phospho-S15) polyclonal antibody（KU）

Swiss-Prot :P04637

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNAPK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage. Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity. p53 is phosphorylated at Ser392 in vivo and by CAK in vitro. Phosphorylation of p53 at Ser392 is altered in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-p53 (S15) polyclonal antibody detects endogenous levels of p53 protein only when phosphorylated at Ser15

BiowMW :~ 53 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Hypoxia Signaling,Warburg Effect,Signaling Pathways Activating p38 MAP KinaseCell Cycle Control G1 S Checkpoint,Examples of Crosstalk Between Post-translational Modifications,Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53

Immunogen :Synthetic phosphopeptide derived from human p53 around the phosphorylation site of Serine 15.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-p53 (S15) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with colchicine(0.2ng/ml,24h) Lane2:Raw264.7 cell lysate treated with colchicine(0.2ng/ml,24h) Lane3:H9C2 cell lysate treated with colchicine(0.2ng/ml,24h)

Immunohistochemistry (IHC) analyzes of p-p53 (S15) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Western blot (WB) analysis of p-p53 (S15) pAb at 1:500 dilution Lane1:HEK293T treated with Etoposide(20ng/ml) for 2 hours whole cell lysate Lane2:HEK293T treated with Etoposide(20ng/ml) for 30 minutes whole cell lysate Lane3:HEK293T treated with Etoposide(20ng/ml) for 5 minutes whole cell lysate Lane4:HEK293T whole cell lysate(40ug)

Immunoprecipitation of HepG2 cell lysates using p53 (phospho-S15) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using p53 (phospho-S15) pAb.

MEHP-induced oxidative DNA damage and apoptosis in HepG2 cells correlates with p53-mediated mitochondria-dependent signaling pathway

JournalFood And Chemical Toxicology

ApplicationWB

IF4.679

SpeciesHuman

PMID22542555
Phosphorylated Hsp27 activates ATM-dependent p53 signaling and mediates the resistance of MCF-7 cells to doxorubicin-induced apoptosis

JournalCellular Signalling

ApplicationWB

IF3.388

SpeciesHuman

PMID23357534
MicroRNA-145 Protects Cardiomyocytes against Hydrogen Peroxide (H2O2)-Induced Apoptosis through Targeting the Mitochondria Apoptotic Pathway

JournalPLoS One

ApplicationWB

IF2.74

SpeciesRat

PMID23028672
HSP27 Alleviates Cardiac Aging in Mice via a Mechanism Involving Antioxidation and Mitophagy Activation

JournalOxidative Medicine and Cellular Longevity

ApplicationWB

IF5.076

SpeciesMouse

PMID27110324
Folic acid delays age-related cognitive decline in senescence-accelerated mouse prone 8: alleviating telomere attrition as a potential mechanism

JournalAging-US

ApplicationWB

IF4.831

SpeciesMouse

PMID31757935