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APAF1 Rabbit monoclonal antibody（KU）

货号: BS40093

价格：￥ 1200

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS40093

Product Name :APAF1 Rabbit monoclonal antibody（KU）

Swiss-Prot :O14727

Host :Rabbit

Reactivity :Human Mouse

Applications :WB IHC ICC/IF

Application_all :WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200

Background :APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Induced by E2F and p53 in apoptotic neurons. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. 6 isoforms of the human protein are produced by alternative splicing.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :Affinity-chromatography

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :APAF1 Antibody detects endogenous levels of total APAF1

BiowMW :~ 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413

Immunogen :A synthesized peptide derived from human APAF1

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of APAF1 Rabbit monoclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(30ug) Lane2:HepG2 whole cell lysate(30ug)

Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody .