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IP6K2 (L188) polyclonal antibody（KU）

货号: BS2759

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2759

Product Name :IP6K2 (L188) polyclonal antibody（KU）

Swiss-Prot :Q9UHH9

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The members of the inositol hexakisphosphate kinase family, IP6K1 and IP6K2, have a high affinity and selectivity for inositol hexakisphosphate (InsP6) as a substrate. IP6K1 and IP6K2 (also designated PiUS) convert InsP6 to PP-InsP5. However, neither kinase demonstrates any catalytic activity with other inositol pyrophosphates. The presence of InsP6, which inhibits serine/threonine protein phosphatases, increases the influx of calcium across the plasma membrane and implies that it may mediate the regulation of insulin exocytosis. IP6K1 was purified as a 54 kDa protein in rat brain extracts. By homology, IP6K1 and IP6K2 were characterized in mouse as a 50 kDa and 49 kDa protein, respectively. IP6K1 displays ATP synthase activity by transferring a phosphate from PP-InsP5 to ADP, which suggests a role for the IP6 kinases as high energy phosphate donors.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IP6K2 (L188) polyclonal antibody detects endogenous levels of IP6K2 protein.

BiowMW :~ 52 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Inositol hexakisphosphate kinase 2; InsP6 kinase 2; P(i)-uptake stimulator; PiUS; IP6K2; IHPK2; TCCCIA00113

Immunogen :Synthetic peptide, corresponding to amino acids 156-204 of Human IP6K2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IP6K2 (L188) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of IP6K2 (L188) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of IP6K2 (L188) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.