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MYH (Y176) polyclonal antibody（KU）

货号: BS2535

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2535

Product Name :MYH (Y176) polyclonal antibody（KU）

Swiss-Prot :Q9UIF7

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MYH (Y176) polyclonal antibody detects endogenous levels of MYH protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :A/G-specific adenine DNA glycosylase; MutY homolog; hMYH; MUTYH; MYH

Immunogen :Synthetic peptide, corresponding to amino acids 142-190 of Human MYH.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MYH (Y176) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of MYH (Y176) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

The Polymorphic AluYb8 Insertion in the MUTYH Gene is Associated with Reduced Type 1 Protein Expression and Reduced Mitochondrial DNA Content

JournalPLoS One

ApplicationWB

IF2.776

SpeciesHuman

PMID23936466
Chronic exposure to microcystin-LR affected mitochondrial DNA maintenance and caused pathological changes of lung tissue in mice

JournalEnvironmental Pollution

ApplicationWB

IF6.792

SpeciesMouse

PMID26706032
Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells

JournalOxidative Medicine and Cellular Longevity

ApplicationIF,WB

IF5.076

SpeciesMouse,Human

PMID26576226
AluYb8 insertion polymorphism in the MUTYH gene impairs mitochondrial DNA maintenance and affects the age of onset of IPF

JournalAging-US

ApplicationWB

IF5.682

SpeciesMouse,Human

PMID30716719
Atorvastatin ameliorates the contractile dysfunction of the aorta induced by organ culture

JournalNaunyn-Schmiedebergs Archives Of Pharmacology

ApplicationWB

IF2.05

SpeciesRat

PMID30182188