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Cdc20 (Q105) polyclonal antibody（KU）

货号: BS2203

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2203

Product Name :Cdc20 (Q105) polyclonal antibody（KU）

Swiss-Prot :Q12834

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins,Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred..

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cdc20 (Q105) polyclonal antibody detects endogenous levels of Cdc20 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cell division cycle protein 20 homolog; p55CDC; CDC20

Immunogen :Synthetic peptide, corresponding to amino acids 80-130 of Human Cdc20.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Cdc20 (Q105) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:Rat liver tissue lysate

Immunohistochemistry (IHC) analyzes of Cdc20 (Q105) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Cdc20 (Q105) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell Lymphoma

JournalOncoTargets and Therapy

ApplicationWB, IHC

IF3.337

SpeciesHuman

PMID33116627
MTA1 localizes to the mitotic spindle apparatus and interacts with TPR in spindle assembly checkpoint regulation

JournalBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ApplicationWB

IF3.1

SpeciesHuman

PMID37467663