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PERK (R87) polyclonal antibody（KU）

货号: BS2156

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2156

Product Name :PERK (R87) polyclonal antibody（KU）

Swiss-Prot :Q9NZJ5

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2α, thus inhibiting the translation of mRNA.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PERK (R87) polyclonal antibody detects endogenous levels of PERK protein.

BiowMW :~ 140 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral Regulation of elF2,

Alternative Name :Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK

Immunogen :Synthetic peptide, corresponding to amino acids 56-102 of Human PERK.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PERK (R87) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate(20ug) Lane2:MCF-7 whole cell lysate(20ug) Lane3:A375 whole cell lysate(20ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PMVEC whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Inhibition of hepatocyte nuclear factor 1b induces hepatic steatosis through DPP4/NOX1-mediated regulation of superoxide

JournalFree Radical Biology And Medicine

ApplicationWB

IF5.657

SpeciesMouse

PMID28942246
TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity

JournalOxidative Medicine and Cellular Longevity

ApplicationWB

IF4.868

SpeciesRat

PMID28303170
Melatonin, a novel selective ATF-6 inhibitor, induces human hepatoma cell apoptosis through COX-2 downregulation

JournalWorld Journal Of Gastroenterology

ApplicationWB

IF3.665

SpeciesHuman

PMID28246472
E platinum, a newly synthesized platinum compound, induces apoptosis through ROS‐triggered ER stress in gastric carcinoma cells

JournalMolecular Carcinogenesis

ApplicationWB

IF3.411

SpeciesHuman

PMID27061377
Endoplasmic reticulum stress and apoptosis via PERK-eIF2α-CHOP signaling in the methamphetamine-induced chronic pulmonary injury

JournalEnvironmental Toxicology And Pharmacology

ApplicationWB

IF3.061

SpeciesRat

PMID28081472