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PERK (R87) polyclonal antibody（KU）

货号: BS2156

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS2156

Product Name :PERK (R87) polyclonal antibody（KU）

Swiss-Prot :Q9NZJ5

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :An interferon-inducible, RNA-dependent protein serine /threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. The serine /threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. PERK is a type I transmembrane protein located in the endoplasmic reticulum (ER) that contains a kinase domain similar to the kinase domain of PKR. PERK is activated in response to ER stress and phosphorylates eIF-2α, thus inhibiting the translation of mRNA.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PERK (R87) polyclonal antibody detects endogenous levels of PERK protein.

BiowMW :~ 140 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral Regulation of elF2,

Alternative Name :Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK; EIF2AK3; PEK; PERK

Immunogen :Synthetic peptide, corresponding to amino acids 56-102 of Human PERK.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PERK (R87) pAb at 1:1000 dilution Lane1:HCT116 whole cell lysate(20ug) Lane2:MCF-7 whole cell lysate(20ug) Lane3:A375 whole cell lysate(20ug) Lane4:CT26 whole cell lysate(40ug) Lane5:PMVEC whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of PERK (R87) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Endoplasmic Reticulum Stress Promotes Sorafenib Resistance via miR-188-5p/hnRNPA2B1 mediated upregulation of PKM2 in Hepatocellular Carcinoma

JournalMolecular Therapy-Nucleic Acids

ApplicationWB,IHC

IF8.886

SpeciesHuman

PMID34786210
KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells

JournalOncogene

ApplicationWB

IF6.634

SpeciesHuman,Rat

PMID29520102
Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206 + M2-like macrophage polarization

JournalCell Death & Disease

ApplicationWB

IF6.304

SpeciesMouse

PMID32075954
Melatonin Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib through the PERK-ATF4-Beclin1 Pathway

JournalInternational Journal of Biological Sciences

ApplicationWB

IF4.858

SpeciesHuman

PMID31523192
GanDouLing combined with Penicillamine improves cerebrovascular injury via PERK/eIF2α/CHOP endoplasmic reticulum stress pathway in the mouse model of Wilson’s disease

JournalBioscience Reports

ApplicationWB

IF2.535

SpeciesRat

PMID30181379