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TNF-α (Q178) polyclonal antibody

货号: BS1857

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1857

Product Name :TNF-α (Q178) polyclonal antibody

Swiss-Prot :P01375

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 a band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 a protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TNFα (Q178) polyclonal antibody detects endogenous levels of TNFα protein.

BiowMW :~ 17, 26 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Necrotic Cell Death,

Alternative Name :Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, soluble form; TNF; TNFA; TNFSF2; TNFα; TNF α

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of TNF-α expression in HEK293T (A), Hela (B), mouse lung (C), mouse kidney (D), mouse spleen (E) whole cell lysates.

Immunohistochemical analysis of TNF-α staining in human tonsil formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of TNF-α staining in HepG2 cells.

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