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TNF-α (Q178) polyclonal antibody

货号: BS1857

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1857

Product Name :TNF-α (Q178) polyclonal antibody

Swiss-Prot :P01375

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 a band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 a protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TNFα (Q178) polyclonal antibody detects endogenous levels of TNFα protein.

BiowMW :~ 17, 26 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Necrotic Cell Death,

Alternative Name :Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Tumor necrosis factor, soluble form; TNF; TNFA; TNFSF2; TNFα; TNF α

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TNF alpha.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of TNF-α expression in HEK293T (A), Hela (B), mouse lung (C), mouse kidney (D), mouse spleen (E) whole cell lysates.

Immunohistochemical analysis of TNF-α staining in human tonsil formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of TNF-α staining in HepG2 cells.

PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production

JournalCell Death And Differentiation

ApplicationIF

IF15.828

SpeciesMouse

PMID33934104
Retinoblastoma protein promotes uterine epithelial cell cycle arrest and necroptosis for embryo invasion

JournalEmbo Reports

ApplicationIHC

IF8.807

SpeciesMouse

PMID33399260
The Causal Role of Magnesium Deficiency in the Neuroinflammation, Pain Hypersensitivity and Memory/Emotional Deficits in Ovariectomized and Aged Female Mice

JournalJournal of Inflammation Research

ApplicationWB

IF6.922

SpeciesMouse

PMID34908863
DNA binding protein HMGB1 secreted by activated microglia promotes the apoptosis of hippocampal neurons in diabetes complicated with OSA

JournalBrain Behavior And Immunity

ApplicationWB

IF6.17

SpeciesMouse

PMID29920330
Allergic Conjunctivitis-induced Retinal Inflammation Promotes Myopia Progression

JournalEBioMedicine

ApplicationIHC

IF5.736

SpeciesRat

PMID29398596