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Vimentin (L79) polyclonal antibody

货号: BS1855

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1855

Product Name :Vimentin (L79) polyclonal antibody

Swiss-Prot :P08670

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Vimentin (L79) polyclonal antibody detects endogenous levels of Vimentin protein.

BiowMW :~ 57 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :Vimentin; VIM

Immunogen :Synthetic peptide, corresponding to amino acids 31-80 of Human Vimentin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Vimentin (L79) pAb at 1:1000 dilution Lane1:The testis tissue lysate of Rat(40ug) Lane2:The kidney tissue lysate of Mouse(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug) Lane5:Jurkat whole cell lysate (40ug) Lane6:PC3 whole cell lysate (40ug)

Immunohistochemistry (IHC) analyzes of Vimentin (L79) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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