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Wnt-1 (G331) polyclonal antibody（KU）

货号: BS1777

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1777

Product Name :Wnt-1 (G331) polyclonal antibody（KU）

Swiss-Prot :P04628

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein.

BiowMW :~ 41 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1; INT1; WNT 1

Immunogen :Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Wnt-1 (G331) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:DLD whole cell lysate(40ug) Lane4:The Testis tissue lysate of Mouse(40ug) Lane5:The Testis tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of Wnt-1 (G331) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Role of Wnt1 and Fzd1 in the spinal cord pathogenesis of amyotrophic lateral sclerosis-transgenic mice

JournalBiotechnology Letters

ApplicationWB

IF1.977

SpeciesMouse

PMID23553522
Sox9 regulates hyperexpression of Wnt1 and Fzd1 in human osteosarcoma tissues and cells

JournalInternational Journal of Clinical and Experimental Pathology

ApplicationIHC,WB,IF

IF0.205

SpeciesHuman

PMID25197350
Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately

JournalStem Cell Research & Therapy

ApplicationWB

IF4.627

SpeciesRat

PMID26076648
Endothelial‑to‑mesenchymal transition in human idiopathic dilated cardiomyopathy

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID29115553
Penta-acetyl Geniposide Suppresses Migration, Invasion, and Inflammation of TNF-α-Stimulated Rheumatoid Arthritis Fibroblast-Like Synoviocytes Involving Wnt/β-Catenin Signaling Pathway

JournalInflammation

ApplicationWB

IF4.092

SpeciesRat,Human

PMID