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Wnt-1 (G331) polyclonal antibody（KU）

货号: BS1777

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1777

Product Name :Wnt-1 (G331) polyclonal antibody（KU）

Swiss-Prot :P04628

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Products of the highly conserved Wnt gene family play key roles in regulating cellular growth and differentiation. The prototype member of the Wnt gene family, Wnt-1, is a cysteine-rich secreted glycoprotein that associates with cell membranes and likely functions as a key regulator of cellular adhesion. β-catenin, a cadherin-binding cellular adhesion protein which also binds the tumor supressor gene APC, has been identified as a downstream target of a signal transduction pathway mediated by Wnt-1. Wnt-1 is essential for normal development of the embryonic nervous system and its expression is normally limited to the embryonic neural tube and adult spermatids. When improperly expressed in mammary tissue, Wnt-1 contributes to hyperplasia and tumorigenic progression. Wnt family members have been shown to interact with Sonic hedgehog (Shh) in vivo to induce myogenesis in somitic tissue.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Wnt-1 (G331) polyclonal antibody detects endogenous levels of Wnt-1 protein.

BiowMW :~ 41 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1; INT1; WNT 1

Immunogen :Synthetic peptide, corresponding to amino acids 301-350 of Human Wnt-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Wnt-1 (G331) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:DLD whole cell lysate(40ug) Lane4:The Testis tissue lysate of Mouse(40ug) Lane5:The Testis tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of Wnt-1 (G331) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

DNMT1-Induced miR-152-3p Suppression Facilitates Cardiac Fibroblast Activation in Cardiac Fibrosis

JournalCardiovascular Toxicology

ApplicationWB

IF3.231

SpeciesRat

PMID34424481
Umbelliferone Inhibits Migration, Invasion and Inflammation of Rheumatoid Arthritis Fibroblast-Like Synoviocytes and Relieves Adjuvant-Induced Arthritis in Rats by Blockade of Wnt/β-Catenin Signaling Pathway

JournalAMERICAN JOURNAL OF CHINESE MEDICINE

ApplicationWB

IF6.005

SpeciesRat，Human

PMID35997647
Restorative effects and mechanisms of neural stem cell transplantation on ischemic brain injury based on the Wnt signaling pathway

JournalINTERNATIONAL JOURNAL OF NEUROSCIENCE

ApplicationWB

IF2.2

SpeciesMouse

PMID38108312
Evidence of the “hit and run” characteristics of Cerebroprotein Hydrolysate-I in the treatment of neonatal HIE based on pharmacokinetic and pharmacological studies

Journal10.1016/j.intimp.2024.113580

ApplicationWB

IF4.8

SpeciesRat

PMID39547013
Sang-Bai-Pi extract and its constituent regiafuran C ameliorate renal fibrosis through TGF-β/Smad and Wnt/β-catenin signaling pathways

Journal10.1016/j.phymed.2024.156351

ApplicationWB

IF6.7

SpeciesHuman,Rat

PMID39756310