产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Syk (P342) polyclonal antibody（KU）

货号: BS1428

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1428

Product Name :Syk (P342) polyclonal antibody（KU）

Swiss-Prot :P43405

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:2000~1:5000 IHC: 1:50~1:200 IF: 1:100~1:500 IP: 1:50~1:200

Background :Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells. Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors. It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas. Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl. Tyr352 of Syk is involved in the association of PLCγ1. Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525/526 of human Syk (equivalent to Tyr519/520 of mouse Syk) is essential for Syk function.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Syk (P342) polyclonal antibody detects endogenous levels of Syk protein.

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,B cell receptor signaling,

Alternative Name :Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK

Immunogen :Synthetic peptide, corresponding to Human Syk.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Syk (P342) polyclonal antibody at 1:2000 dilution Lane1:A431 whole cell lysate(30ug) Lane2:HEK293T whole cell lysate(30ug) Lane3:THP-1 whole cell lysate(30ug) Lane4:PMVEC whole cell lysate(30ug) Lane5:CT-26 whole cell lysate(30ug)

Immunohistochemistry (IHC) analyzes of SYK (P342) pAb in paraffin-embedded human breast carcinoma tissue.

Immunofluorescence analysis of PMVEC cells using Syk (P342) pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of PMVEC cell lysates using Syk (P342) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Syk (P342) pAb.