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PDGF-B (L48) polyclonal antibody（KU）

货号: BS1290

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1290

Product Name :PDGF-B (L48) polyclonal antibody（KU）

Swiss-Prot :P01127

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PDGFB (L48) polyclonal antibody detects endogenous levels of PDGFB protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin; PDGFB; PDGF2; SIS

Immunogen :Synthetic peptide, corresponding to amino acids 21-70 of Human PDGF-B.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PDGF-B (L48) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:3T3-L1 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PDGF-B (L48) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Hypoxia-Inducible Factor Activation in Myeloid Cells Contributes to the Development of Liver Fibrosis in Cholestatic Mice

JournalJournal Of Pharmacology And Experimental Therapeutics

ApplicationIHC

IF4.03

SpeciesMouse

PMID22271822
Implication of PDGF signaling in cigarette smoke-induced pulmonary arterial hypertension in rat

JournalInhalation Toxicology

ApplicationWB

IF2.724

SpeciesRat

PMID
Seasonal Changes in Morphology and Immunoreactivity of PDGF-A and its Receptor PDGFR-α in the Epididymis of Wild Ground Squirrels (Citellus dauricus Brandt)

JournalJournal Of Reproduction And Development

ApplicationIHC

IF2.214

SpeciesRat

PMID
Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling

JournalJournal Of Biomedicine And Biotechnology

ApplicationWB

IF0

SpeciesRat

PMID22754279
Prognostic significance of platelet-derived growth factor receptor-β expression in localized clear cell renal cell carcinoma

JournalJournal Of Cancer Research And Clinical Oncology

ApplicationIHC

IF3.656

SpeciesHuman

PMID26215891