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PDGF-B (L48) polyclonal antibody（KU）

货号: BS1290

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1290

Product Name :PDGF-B (L48) polyclonal antibody（KU）

Swiss-Prot :P01127

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, α and β, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to α receptors with high affinity, whereas the B subunit can bind to both α and β receptors. Evidence suggests that PDGF may function as a neurotrophic factor.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PDGFB (L48) polyclonal antibody detects endogenous levels of PDGFB protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis; Becaplermin; PDGFB; PDGF2; SIS

Immunogen :Synthetic peptide, corresponding to amino acids 21-70 of Human PDGF-B.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PDGF-B (L48) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:3T3-L1 whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:H1792 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PDGF-B (L48) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

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