产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

E-Cadherin (R868) polyclonal antibody （KU）

货号: BS1098

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1098

Product Name :E-Cadherin (R868) polyclonal antibody （KU）

Swiss-Prot :P12830/P19022/P22223/P55283

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Background :Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :E-cadherin (R868) polyclonal antibody detects endogenous levels of E-cadherin protein.

BiowMW :~ 98, 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,Contribution of ECM and Cytoskeletal Factors to EMT,Contribution of Soluble Factors to EMT,Adherens Junction signaling,

Alternative Name :Cadherin-1; Cadherin1; Cadherin 1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDH1; CDHE; UVO

Immunogen :Synthetic peptide, corresponding to amino acids 840-880 of Human E-cadherin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of E-cadherin (R868) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of E-Cadherin (R868) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of HepG2 cell lysate using E-cadherin (R868) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using E-cadherin (R868) #BS1098. “↑”（supernatant）; “↓(deposition）

Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma

JournalOncogene

ApplicationIF,IHC,WB

IF7.971

SpeciesHuman,Mouse

PMID26549030
Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-β/β-catenin signaling and epithelial–mesenchymal transition

JournalCancer Letters

ApplicationWB

IF6.508

SpeciesHuman

PMID25449432
Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-

JournalBreast Cancer Research

ApplicationIHC,WB,IF

IF5.676

SpeciesHuman

PMID25990368
HBx protein‐mediated ATOH1 downregulation suppresses ARID2 expression and promotes hepatocellular carcinoma

JournalCancer Science

ApplicationWB

IF4.966

SpeciesHuman

PMID28498550
AEG‐1 induces gastric cancer metastasis by upregulation of eIF4E expression

JournalJournal Of Cellular And Molecular Medicine

ApplicationWB

IF4.658

SpeciesHuman

PMID28661037