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E-Cadherin (R868) polyclonal antibody （KU）

货号: BS1098

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1098

Product Name :E-Cadherin (R868) polyclonal antibody （KU）

Swiss-Prot :P12830/P19022/P22223/P55283

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF IP

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Background :Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell adhesion. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual cadherin molecules are known to co-operate with each other to form a linear cell adhesion zipper. In adhesion junctions cadherins are bound to beta and gamma catenins which in turn bind to alpha catenin, an actin binding protein. Cadherins play an important part in tumor invasion and metastasis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :E-cadherin (R868) polyclonal antibody detects endogenous levels of E-cadherin protein.

BiowMW :~ 98, 130 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Neuronal and Glial Cell Marker Atlas,Contribution of ECM and Cytoskeletal Factors to EMT,Contribution of Soluble Factors to EMT,Adherens Junction signaling,

Alternative Name :Cadherin-1; Cadherin1; Cadherin 1; CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; CDH1; CDHE; UVO

Immunogen :Synthetic peptide, corresponding to amino acids 840-880 of Human E-cadherin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of E-cadherin (R868) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of E-Cadherin (R868) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of HepG2 cell lysate using E-cadherin (R868) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ) .Lane 1 is 30% input. The western blot was probed using E-cadherin (R868) #BS1098. “↑”（supernatant）; “↓(deposition）

Hepatitis C virus core protein epigenetically silences SFRP1 and enhances HCC aggressiveness by inducing epithelial–mesenchymal transition

JournalOncogene

ApplicationWB

IF7.971

SpeciesHuman

PMID
Mycoplasma hyorhinis induces epithelial-mesenchymal transition in gastric cancer cell MGC803 via TLR4-NF-κB signaling

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IF7.36

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PMID25149064
Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway

JournalInternational Journal Of Cancer

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IF5.145

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PMID24374650
Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF‐β signalling

JournalJournal Of Cellular And Molecular Medicine

ApplicationIHC,WB

IF4.658

SpeciesHuman

PMID24912879
SASH1 regulates melanocyte transepithelial migration through a novel Gαs–SASH1–IQGAP1–E-Cadherin dependent pathway

JournalCellular Signalling

ApplicationIHC,WB

IF3.968

SpeciesHuman

PMID23333244