产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

CREB (I127) polyclonal antibody（KU）

货号: BS1077

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1077

Product Name :CREB (I127) polyclonal antibody（KU）

Swiss-Prot :P16220

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CREB(I127) polyclonal antibody detects endogenous levels of CREB protein.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human CREB.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CREB (I127) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes ofCREB (I127) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Downregulation of prostaglandin E2 is involved in hindgut mucosal damage in lactating goats fed a high‐concentrate diet

JournalExperimental Physiology

ApplicationWB

IF2.431

SpeciesGoat

PMID26593381
ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice

JournalExperimental Neurology

ApplicationWB

IF4.562

SpeciesMouse

PMID30201537
The anti-androgenic effects of cypermethrin mediated by non-classical testosterone pathway activation of mitogen-activated protein kinase cascade in mouse Sertoli cells

JournalEcotoxicology And Environmental Safety

ApplicationWB

IF4.527

SpeciesMouse

PMID30974244
Neuroprotective effects of novel compound Tozan on cognition, neurogenesis and apoptosis in diabetes

JournalAnnals of Translational Medicine

ApplicationWB

IF3.932

SpeciesMouse,Human

PMID34734023
Activation of ERα and/or ERβ ameliorates cognitive impairment and apoptosis in streptozotocin-induced diabetic mice

JournalHormones And Behavior

ApplicationWB

IF3.684

SpeciesMouse

PMID30096284