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CREB (I127) polyclonal antibody（KU）

货号: BS1077

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1077

Product Name :CREB (I127) polyclonal antibody（KU）

Swiss-Prot :P16220

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CREB(I127) polyclonal antibody detects endogenous levels of CREB protein.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1; CREB1

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human CREB.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CREB (I127) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes ofCREB (I127) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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