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SQSTM1 polyclonal antibody（KU）

货号: AP6006

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :AP6006

Product Name :SQSTM1 polyclonal antibody（KU）

Swiss-Prot :Q13501

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB: 1:500~1:1000 IF: 1:50~1:200 IHC: 1:50~1:200 IP: 1:50~1:200

Background :The chronic focal skeletal disorder, Paget’s disease of bone, affects 2-3% of the population over the age of 60 years. Paget’s disease is characterized by increased bone resorption by osteoclasts, followed by abundant new bone formation that is of poor quality. The disease leads to several complications including bone pain and deformities, as well as fissures and fractures. Mutations in the ubiquitin-associated (UBA) domain of the Sequestosome 1 protein (SQSTM1), also designated p62 or ZIP, commonly cause Paget’s disease since the UBA is necessary for aggregate sequestration and cell survival.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SQSTM1 polyclonal antibody detects endogenous levels of SQSTM1 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA; OSIL

Immunogen :Recombinant full length Human SQSTM1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SQSTM1 polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:NIH-3T3 whole cell lysate(30μg) Lane3:PC12 whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of SQSTM1 pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasm staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Western blot (WB) analysis of SQSTM1 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(human) Lane3:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(Anti-SQSTM1, IP) Lane4:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(Anti-SQSTM1, IP,supernatant) Lane5:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(Anti-Flag, IP)

Immunofluorescence analysis of U-8MG cells using SQSTM1 antibody at dilution of 1:50.

Lycopene prevents carcinogen-induced cutaneous tumor by enhancing activation of the Nrf2 pathway through p62-triggered autophagic Keap1 degradation

JournalAging-US

ApplicationWB

IF4.831

SpeciesMouse

PMID32365333
Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model

JournalNeural Regeneration Research

ApplicationWB

IF3.171

SpeciesRat,Human

PMID33433498
Ginsenoside metabolite 20(S)‑protopanaxadiol promotes neural stem cell transition from a state of proliferation to differentiation by inducing autophagy and cell cycle arrest

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesRat

PMID32319663
Intraneuronal sortilin aggregation relative to granulovacuolar degeneration, tau pathogenesis and sorfra plaque formation in human hippocampal formation

JournalFrontiers in Aging Neuroscience

ApplicationIHC,IF

IF5.702

SpeciesHuman

PMID35978952
Around-the-Clock Noise Induces AD-like Neuropathology by Disrupting Autophagy Flux Homeostasis

JournalCells

ApplicationWB

IF7.666

SpeciesRat

PMID36078149