产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

SQSTM1 polyclonal antibody（KU）

货号: AP6006

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP6006

Product Name :SQSTM1 polyclonal antibody（KU）

Swiss-Prot :Q13501

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB: 1:500~1:1000 IF: 1:50~1:200 IHC: 1:50~1:200 IP: 1:50~1:200

Background :The chronic focal skeletal disorder, Paget’s disease of bone, affects 2-3% of the population over the age of 60 years. Paget’s disease is characterized by increased bone resorption by osteoclasts, followed by abundant new bone formation that is of poor quality. The disease leads to several complications including bone pain and deformities, as well as fissures and fractures. Mutations in the ubiquitin-associated (UBA) domain of the Sequestosome 1 protein (SQSTM1), also designated p62 or ZIP, commonly cause Paget’s disease since the UBA is necessary for aggregate sequestration and cell survival.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SQSTM1 polyclonal antibody detects endogenous levels of SQSTM1 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA; OSIL

Immunogen :Recombinant full length Human SQSTM1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SQSTM1 polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:NIH-3T3 whole cell lysate(30μg) Lane3:PC12 whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of SQSTM1 pAb in paraffin-embedded human lung carcinoma tissue at 1:50,showing cytoplasm staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Western blot (WB) analysis of SQSTM1 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate ,untransfected Lane2:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(human) Lane3:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(Anti-SQSTM1, IP) Lane4:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(Anti-SQSTM1, IP,supernatant) Lane5:HEK293T whole cell lysate , transfected with pcDNA3.1-HA-FlaG-p62(Anti-Flag, IP)

Immunofluorescence analysis of U-8MG cells using SQSTM1 antibody at dilution of 1:50.

Asymptomatic Hyperuricemia Is Associated with Achilles Tendon Rupture through Disrupting the Normal Functions of Tendon Stem/Progenitor Cells

JournalStem Cells International

ApplicationWB

IF5.131

SpeciesRat

PMID36504525
CDK4/6 Inhibitors Impede Chemoresistance and Inhibit Tumor Growth of Small Cell Lung Cancer

JournalAdvanced Science

ApplicationWB

IF14.3

SpeciesHuman

PMID39136283
Leptin promotes tendon stem/progenitor cell senescence through the AKT-mTOR signaling pathway

Journal10.1016/j.yexcr.2024.114274

ApplicationWB

IF3.3

SpeciesRat

PMID39393753