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LC3 (T50) polyclonal antibody（KU）

货号: AP1013

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP1013

Product Name :LC3 (T50) polyclonal antibody（KU）

Swiss-Prot :Q9GZQ8/Q9H492/Q9BXW4

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LC3 (T50) polyclonal antibody detects endogenous levels of LC3-I and LC3-II protein.

BiowMW :~ 15 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phosphoinosotode Signaling,Autophagy Signaling,

Alternative Name :Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II

Immunogen :A synthetic peptide corresponding to residues in Human LC3.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of LC3 (T50) polyclonal antibody at 1:500 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:SK-OVCAR3 whole cell lysate(10ug) Lane4:L02 whole cell lysate(10ug) Lane5:HEK293T whole cell lysate(40ug)

Western blot (WB) analysis of LC3 (T50) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T transfected with pEGFP-C3 whole cell lysate(10ug) Lane3:HEK293T transfected with pEGFP-C3-LC3B whole cell lysate(10ug)

Chronic intermittent hypoxia induces cardiac hypertrophy by impairing autophagy through the adenosine 5′-monophosphate-activated protein kinase pathway

JournalArchives Of Biochemistry And Biophysics

ApplicationWB

IF3.559

SpeciesRat

PMID27412517
Paeonol Inhibits Oxidized Low-Density Lipoprotein-Induced Vascular Endothelial Cells Autophagy by Upregulating the Expression of miRNA-30a

JournalFrontiers in Pharmacology

ApplicationWB

IF3.845

SpeciesRat

PMID29472864
SHP-2 restricts apoptosis induced by chemotherapeutic agents via Parkin-dependent autophagy in cervical cancer

JournalCancer Cell International

ApplicationWB

IF3.439

SpeciesHuman

PMID29371831
Neuroprotective effects of the novel GLP-1 long acting analogue semaglutide in the MPTP Parkinson's disease mouse model

JournalNeuropeptides

ApplicationWB

IF2.407

SpeciesMouse

PMID30017231