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ATG2B (D2078) polyclonal antibody（KU）

货号: AP0776

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0776

Product Name :ATG2B (D2078) polyclonal antibody（KU）

Swiss-Prot :Q96BY7

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IP IHC

Application_all :WB: 1:500~1:1000 IP: 1:50~1:200 IHC: 1:50~1:200

Background :ATG2B,required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG2B (D2078) polyclonal antibody detects endogenous levels of ATG2B protein.

BiowMW :~ 300 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Autophagy-related protein 2 homolog B; ATG2B

Immunogen :Synthetic peptide, corresponding to Human ATG2B.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of ATG2B (D2078) polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug)

Immunoprecipitation of 293T cell lysates using ATG2B (D2078) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using ATG2B (D2078) pAb #AP0776.

Immunohistochemistry of paraffin-embedded Human Ovarian cancer using ATG2B antibody at dilution of 1:50.